... very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... the structural and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 domain (B) KIND2 domain anchors v-KIND to dendrites Flag-tagged v-KIND, DKIND1, DKIND2, DKIND1 + 2, DRasN, DGEF, KIND1 or KIND2 together with...
... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms ... C-terminus (box in Fig 1) at sites: 92, 75, 66 and 58 As histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although ... binding on the secondary structure was determined using CD in the far UV To prepare proteins without Cu2+, part of each protein 4252 Fig Comparison of copper binding by stefin A and stefin B using...
... (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations ... were removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation ... were incubated at 37 °C in a humidified 5% CO2 incubator in the absence (control) and presence of increasing concentrations of inhibitors for 10 days, and the number of molting larvae was determined...
... (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK or by defining a new pathway of RTK signaling [8] RTKs play important roles in cell signaling during cell ... proteins, Vpr, has been shown to dysregulate several host cellular functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins ... Oncogenes and Related Genes Shanghai Cancer Institute, Shanghai, China Smith RK, Carroll PM, Allard JD & Simon MA (2000) MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila...
... no membrane-spanning domains in PEX14 have been unambiguously identified and it was observed in mammalian cells and S cerevisiae that both the N-terminal and the C-terminal domain are at the cytosolic ... synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The miscompartmentation of PTS-1 and PTS-2 proteins (GAPDH and ALD, ... approximately 0.5 to induce the expression of the protein and growth was continued for h Cells were collected by centrifugation (3000 g, 10 min, °C) and resuspended in cell lysis buffer containing 50 mM...
... received his B.S and M.S degrees in electrical engineering from Fudan University, Shanghai, China, in 1983 and 1986, respectively, and his Ph.D degree in imaging science from Rochester Institute of ... maximum point of a Point a will belong to the same peak as point b Similarly, point b belongs to the same peak as its local maximum point c and point c belongs to peak Therefore, points a, b, and c ... respectively, n1i and n2 j represent the data point numbers in cluster i of {H1 } and cluster j of {H2 }, respectively, and mi j is the number of data points existing both in cluster i of {H1 } andin cluster...
... also increasingly involved in the development and delivery of a range of education and training programmes designed to help individuals working in HIV advance professionally, from administering ... Providing education and training opportunities for its membership is important for any professional society, and the IAS is looking at ways to strategically expand itsrolein this area in order ... cross-disciplinary linkages and dialogue, and to strengthen the focus on youth – began to be implemented in the planning for the XVI International AIDS Conference (AIDS 2006) in Toronto and will...
... function in the brain BGT-1 is capable of transporting both betaine and GABA, as examined in Xenopus oocytes and various cell lines But the physiological substrate of BGT-1 in the brain remains to ... decrease in GAT-1 and GAT-3 immunostaining, but no change in GAT-2 staining, was observed in the degenerating CA subfields In contrast, increased BGT-1 immunoreactivity was observed in astrocytes ... immunoreactivity in neurons and little induction of BGT-1 in astrocytes, a marked increase in BGT-1 immunoreactivity was observed in astrocytes in the degenerating CA fields and fimbria, in rats injected...
... critical in inducing cell signaling (Farooqui and Horrocks, 2006) The release of AA is mediated by two enzymatic mechanisms – direct mechanism involving PLA2 and an indirect mechanism involving PLC ... neurotoxins, myotoxins, anticoagulants and proinflammatory proteins (Kini and Evans, 1989) The pathological effects of these venom sPLA2s have been attributed to its high binding affinity to sPLA2 specific ... cPLA2 immunolabelling is found in the hindbrain with light immunostaining observed in the forebrain and midbrain (Farooqui et al., 2000c) iPLA2 has been shown to be implicated in neurodegenerative...
... wild-type occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon played a major rolein the induction of mitochondria-mediated apoptosis and the ... occludin in apoptosis and invasion A C B D E F Fig Identification of a human occludin splice variant in liver cell lines (A) Occludin protein expression was examined with an antibody to the N-terminus ... cells In Chang cells, stained occludin was observed in the cytoplasmic region Additionally, in OccWT-overexpressing Hep3B and Huh7 cells, regions stained with antibody to hemagglutinin (HA), indicating...
... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important rolein leucine catabolism AUH was overproduced in E coli and characterized by measuring ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay ... Characterisation and mitochondrial localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase Gene 228, 85–91 Nakagawa J & Moroni C (1997) A 20-amino-acid autonomous RNA-binding domain contained in...
... aB-Crystallin R120 corresponds to aA-crystallin R116 and both are conserved a-crystallin domain arginines R120G aB-crystallin permits intermediate filament self association in vitro, although binding ... opaque with age and contain many inclusion bodies reactive with antibody to aB-crystallin, but not to b- and c-crystallin, suggesting an important role for aA-crystallin in maintaining lens transparency ... formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and small inclusion bodies of huntingtin, a protein possessing abnormal polyQ...
... Printing Office, Washington, D.C., 2004 SOLAR AND SPACE PHYSICS ANDITSROLEIN SPACE EXPLORATION the fundamental role of solar and space physics research both in scientific exploration andin ... principal operating agency of both the National Academy of Sciences and the National Academy of Engineering in providing services to the government, the public, and the scientific and engineering ... Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers It is autonomous inits administration andin the...
... Available online http://arthritis-research.com/content/6/1/26 known roles in tissue inflammation, and damage in innate immunity Besides its capacity to drive the production of IFN-γ and IL-2 by ... Stoll S, Germain RN: Imaging of T-cell interactions with antigen presenting cells in culture andin intact lymphoid tissue Immunol Rev 2002, 189:51-63 O’Sullivan B, Thomas R: CD40 and dendritic ... development of inflammatory arthritis [14-16] This highlights the critical role of IL-1ra in the constitutive maintenance of peripheral tolerance, andin counterbalancing the proinflammatory effects...
... loss during intestinal tumorigenesis may, in part, act through upregulation of b-catenin, increasing intestinal b-catenin signalingand cell proliferation Also, increase in small-intestinal mucosa ... regulating cell cycle genes and interacting with key DNA damage-activated checkpoint proteins Per1 overexpression in cancer cells increases ionizing radiationinduced apoptosis, whereas inhibition ... regulating the circadian clock include acetylation, phosphorylation, ubiquitination and sumoylation In terms of phosphorylation, Casein kinase epsilon (CK1ε) and Casein kinase delta (CK1ε and CK1δ),...
... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... molecular chaperones in the folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342 ... cytokines play a major rolein sepsis-induced liver injury [19,20], we also investigated the expression of PDI in cells of the macrophage-like cell line, RAW 264.7, after incubation with LPS In...
... invasiveness in vitro and tumor metastasis in vivo The N-terminal fibronectin (FN) binding domain of TG2 (tTG 1 -140) , lacking both enzymatic and GTPase function, induced EMT in OC cells, suggesting the interaction ... transduced into OV90 cells 103 xiv Figure 33 Wild-type TG2 and N-terminal fibronectin binding domain of TG2 induce EMT in OV90 cells Figure 34 104 Wild-type TG2 and N-terminal fibronectin binding domain ... at its protease sensitive hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase...
... expressed predominantly in erythroid cells HRI is regulated by heme through the two heme-binding regions in HRI: an N-terminal domain of HRI andin an insert region in the protein kinase domain of HRI ... the initiation codon in underline and bold and a flanking purine at the -3 and a G at the +4 positions (15) Together the eIF1 and eIF1A facilitate the recognition and selection of initiating ... cytoplasmic splicing of mRNA encoding the Xbox binding protein1 (XBP1) in mammals and homologue of the mammalian activating transcription factor/cAMP-response element (CRE)-binding protein (HAC1) in yeast...
... knockdown MIN6 cell line Figure4.2 Increased betagranin storage in BIG3-deficient MIN6 cells Figure4.3 Increased insulin storage in BIG3-deficient MIN6 cells ix Figure4.4 Increased betagranin secretion ... is more predominant ( Reaven, 1993; Ferrannini, 1998; Relimpio, 2003) The balance between insulin action and insulin secretion is coordinated by insulin sensitive tissues and endocrinal beta cells ... protein recruitment distiguishes the cargo-loaded subdomains from the rest of TGN Curvature-inducing proteins, such as the Bin/amphiphysin/Rvs (BAR) domain family and the GRIP-domain-containing...